MersV1, Main, Exploration, bibRecord, 000C54

One-Health: a Safe, Efficient, Dual-Use Vaccine for Humans and Animals against Middle East Respiratory Syndrome Coronavirus and Rabies Virus.

Identifieur interne : 000C54 ( Main/Exploration ); précédent : 000C53; suivant : 000C55

One-Health: a Safe, Efficient, Dual-Use Vaccine for Humans and Animals against Middle East Respiratory Syndrome Coronavirus and Rabies Virus.

Auteurs : Christoph Wirblich [États-Unis] ; Christopher M. Coleman [États-Unis] ; Drishya Kurup [États-Unis] ; Tara S. Abraham [États-Unis] ; John G. Bernbaum [États-Unis] ; Peter B. Jahrling [États-Unis] ; Lisa E. Hensley [États-Unis] ; Reed F. Johnson [États-Unis] ; Matthew B. Frieman [États-Unis] ; Matthias J. Schnell [États-Unis]

Source :

Journal of virology [ 1098-5514 ] ; 2017.

RBID : pubmed:27807241

Descripteurs français

English descriptors

KwdEn :
- Animals, Coronavirus Infections (immunology), Coronavirus Infections (prevention & control), Coronavirus Infections (virology), Cross Protection (immunology), Disease Models, Animal, Gene Expression Regulation, Viral, Humans, Immunization, Mice, Microbial Interactions, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Rabies (immunology), Rabies (prevention & control), Rabies (virology), Rabies virus (genetics), Rabies virus (immunology), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (immunology), Vaccines, Attenuated, Vaccines, Synthetic, Viral Proteins (genetics), Viral Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (adverse effects), Viral Vaccines (genetics), Viral Vaccines (immunology), Virus Assembly.
MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , adverse effects : Viral Vaccines.
- chemical , genetics : Recombinant Fusion Proteins, Spike Glycoprotein, Coronavirus, Viral Proteins, Viral Vaccines.
- genetics : Middle East Respiratory Syndrome Coronavirus, Rabies virus.
- immunology : Coronavirus Infections, Cross Protection, Middle East Respiratory Syndrome Coronavirus, Rabies, Rabies virus, Recombinant Fusion Proteins, Spike Glycoprotein, Coronavirus, Viral Proteins, Viral Vaccines.
- prevention & control : Coronavirus Infections, Rabies.
- virology : Coronavirus Infections, Rabies.
- Animals, Disease Models, Animal, Gene Expression Regulation, Viral, Humans, Immunization, Mice, Microbial Interactions, Vaccines, Attenuated, Vaccines, Synthetic, Virus Assembly.

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012 and is a highly pathogenic respiratory virus. There are no treatment options against MERS-CoV for humans or animals, and there are no large-scale clinical trials for therapies against MERS-CoV. To address this need, we developed an inactivated rabies virus (RABV) that contains the MERS-CoV spike (S) protein expressed on its surface. Our initial recombinant vaccine, BNSP333-S, expresses a full-length wild-type MERS-CoV S protein; however, it showed significantly reduced viral titers compared to those of the parental RABV strain and only low-level incorporation of full-length MERS-CoV S into RABV particles. Therefore, we developed a RABV-MERS vector that contained the MERS-CoV S1 domain of the MERS-CoV S protein fused to the RABV G protein C terminus (BNSP333-S1). BNSP333-S1 grew to titers similar to those of the parental vaccine vector BNSP333, and the RABV G-MERS-CoV S1 fusion protein was efficiently expressed and incorporated into RABV particles. When we vaccinated mice, chemically inactivated BNSP333-S1 induced high-titer neutralizing antibodies. Next, we challenged both vaccinated mice and control mice with MERS-CoV after adenovirus transduction of the human dipeptidyl peptidase 4 (hDPP4) receptor and then analyzed the ability of mice to control MERS-CoV infection. Our results demonstrated that vaccinated mice were fully protected from the MERS-CoV challenge, as indicated by the significantly lower MERS-CoV titers and MERS-CoV and mRNA levels in challenged mice than those in unvaccinated controls. These data establish that an inactivated RABV-MERS S-based vaccine may be effective for use in animals and humans in areas where MERS-CoV is endemic.

DOI: 10.1128/JVI.02040-16
PubMed: 27807241

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012 and is a highly pathogenic respiratory virus. There are no treatment options against MERS-CoV for humans or animals, and there are no large-scale clinical trials for therapies against MERS-CoV. To address this need, we developed an inactivated rabies virus (RABV) that contains the MERS-CoV spike (S) protein expressed on its surface. Our initial recombinant vaccine, BNSP333-S, expresses a full-length wild-type MERS-CoV S protein; however, it showed significantly reduced viral titers compared to those of the parental RABV strain and only low-level incorporation of full-length MERS-CoV S into RABV particles. Therefore, we developed a RABV-MERS vector that contained the MERS-CoV S1 domain of the MERS-CoV S protein fused to the RABV G protein C terminus (BNSP333-S1). BNSP333-S1 grew to titers similar to those of the parental vaccine vector BNSP333, and the RABV G-MERS-CoV S1 fusion protein was efficiently expressed and incorporated into RABV particles. When we vaccinated mice, chemically inactivated BNSP333-S1 induced high-titer neutralizing antibodies. Next, we challenged both vaccinated mice and control mice with MERS-CoV after adenovirus transduction of the human dipeptidyl peptidase 4 (hDPP4) receptor and then analyzed the ability of mice to control MERS-CoV infection. Our results demonstrated that vaccinated mice were fully protected from the MERS-CoV challenge, as indicated by the significantly lower MERS-CoV titers and MERS-CoV and mRNA levels in challenged mice than those in unvaccinated controls. These data establish that an inactivated RABV-MERS S-based vaccine may be effective for use in animals and humans in areas where MERS-CoV is endemic.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Maryland</li>
<li>Pennsylvanie</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Wirblich, Christoph" sort="Wirblich, Christoph" uniqKey="Wirblich C" first="Christoph" last="Wirblich">Christoph Wirblich</name>
</region>
<name sortKey="Abraham, Tara S" sort="Abraham, Tara S" uniqKey="Abraham T" first="Tara S" last="Abraham">Tara S. Abraham</name>
<name sortKey="Bernbaum, John G" sort="Bernbaum, John G" uniqKey="Bernbaum J" first="John G" last="Bernbaum">John G. Bernbaum</name>
<name sortKey="Coleman, Christopher M" sort="Coleman, Christopher M" uniqKey="Coleman C" first="Christopher M" last="Coleman">Christopher M. Coleman</name>
<name sortKey="Frieman, Matthew B" sort="Frieman, Matthew B" uniqKey="Frieman M" first="Matthew B" last="Frieman">Matthew B. Frieman</name>
<name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E" last="Hensley">Lisa E. Hensley</name>
<name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B" last="Jahrling">Peter B. Jahrling</name>
<name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F" last="Johnson">Reed F. Johnson</name>
<name sortKey="Kurup, Drishya" sort="Kurup, Drishya" uniqKey="Kurup D" first="Drishya" last="Kurup">Drishya Kurup</name>
<name sortKey="Schnell, Matthias J" sort="Schnell, Matthias J" uniqKey="Schnell M" first="Matthias J" last="Schnell">Matthias J. Schnell</name>
</country>
</tree>
</affiliations>
</record>

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Serveur d'exploration MERS

One-Health: a Safe, Efficient, Dual-Use Vaccine for Humans and Animals against Middle East Respiratory Syndrome Coronavirus and Rabies Virus.

One-Health: a Safe, Efficient, Dual-Use Vaccine for Humans and Animals against Middle East Respiratory Syndrome Coronavirus and Rabies Virus.

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